Table 3.
Immunofluorescence conditions.
| Primary Antibody | Brand | Dilution | Secondary Antibody |
Brand | Dilution |
|---|---|---|---|---|---|
| Rabbit α-Olig2 | Santa Cruz | 1:200 5% BSA in PBS/0.25% Triton X-100 |
FITC conjugated goat anti-rabbit IgG (H + L) | Jackson ImmunoResearch |
1:200, 5% BSA in PBS/0.25% triton X-100 |
| Mouse α-MBP | Santa Cruz | 1:200 5% BSA in PBS/0.25% Triton X-100 |
TRITC conjugated goat anti-mouse IgG (H + L) |
Jackson ImmunoResearch |
1:200, 5% BSA in PBS/0.25% triton X-100 |
| Mouse α-GFAP | Abcam | 1:1500 5% BSA in PBS/0.25% Triton X-100 |
FITC conjugated goat anti-rabbit IgG (H + L) | Jackson ImmunoResearch |
1:200, 5% BSA in PBS/0.25% triton X-100 |
MBP: myelin basic protein; GFAP: glial fibrillary acidic protein; BSA: bovine serum albumin; FITC: fluorescein isothiocyanate; TRITC: tetramethyl rhodamine; PBS: phosphate buffer saline.