Figure 2.

Cryopreservation steps of the encapsulation-dehydration, encapsulation-vitrification, and droplet-vitrification techniques: (A) preculture of nodal explants on the medium with an increased sucrose and ABA concentration; (B) shoot tip encapsulated in calcium alginate; (C) osmotic dehydration of encapsulated explants in a concentrated sucrose solution; (D) air drying of explants; (E) dehydration in PVS; (F) cryotube with aluminum foil strip and attached shoot tip; (G) cryobank in the Botanical Garden of the Polish Academy of Sciences in Warsaw, Poland; (H) recovery of explants on a cytokinin-supplemented medium. Bar = 1 cm (except for Figure 2(B)—1 mm).