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. 2022 Jun 9;10(6):1358. doi: 10.3390/biomedicines10061358

Figure 3.

Figure 3

Assessment of primed and naive hPSCs (hESCs, hiPSCs) pluripotency in different culture media conditions. (A,B) Immunofluorescence analysis of pluripotent capacity specific markers (OCT4, NANOG and TRA-1-81) on primed and naive hPSCs on feeder and feeder-free cultures. Scale bar, 100 µm. (C) Gene expression analysis for pluripotency specific genes (SSEA4, NANOG, SOX2 and OCT4) on feeder and feeder-free hPSCs cultures. All comparisons are relative to primed media (* p < 0.1, ** p < 0.01 and *** p < 0.001). Data are represented as mean ± SD.