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Conversion of compound 2 to the tripeptide ψ-GSH (1) after administration in mice. Compounds 1 and 2 (250 mg/kg) were injected in 8–10-week old CD1 mice by intraperitoneal route. Quantitation of ψ-GSH (1) formed after injection of 2 (A) and vice versa (B) was performed using LC-MS/MS analysis. Formation of tripeptide 1 from the dipeptide 2 was detected, although at much lower levels.
