Table 2.

Common 2D models used for bidimensional wound healing assays.

2D Models	Aim	Ref.
Keratinocytes monolayers	Study of the migration behavior to reproduce re-epithelialization process	[33,38,39,42,44]
Fibroblasts monolayers	Evaluation of the migratory potential to study their speed, persistence, and polarity during granulation tissue formation	[31,32,35,46]
Endothelial cells	How endothelial cells migrate and grow towards an angiogenic stimulus to form sprouts	[43,45,48,50]
2D wound assay	Description	Ref.
Scratch Assay	Scratch ofa confluent monolayer of cells with pipette tip, cell scrapers, toothpicks or metallic micro indenters	[30,31,32,33,35,36,37,41,42,43,44,50,51]
Stamp Wound Assay	Determine a lesion on the cell culture with a high-pressure force	[38,39]
Thermal injury Assay	Injury a specific zone of the cell monolayer with very high or low temperatures	[45]
Electrical Injury Assay	Destruction of a cell portion by applying an electric current	[46,47,48]
Optical Injury Assay	Formation of a wounded area by means of a laser beam	[49]