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. 2022 Jun 8;10(6):1352. doi: 10.3390/biomedicines10061352

Figure 4.

Figure 4

Citrate shows p53-independent inhibitory effects on HCT116 cells. (A) HCT116 WT or HCT116 p53 (-/-) cells were treated with the indicated concentrations of citrate for 72 h. Cell growth was measured using a Cell Counting Kit-8 assay. (B) HCT116 p53 (-/-) cells were treated with the indicated concentrations of Citrus limon L.-derived nanovesicles for 6 h. Intracellular pH was measured using a BCECF-AM assay. Data are means ± standard error measurement; n = 3; * p < 0.05, relative to the PBS (-) control. p values were calculated using Dunnett’s multiple comparisons test and Sidak’s multiple comparisons test.