Figure 5.

Pharmacological modulation of zMc4r signaling by RMrap2a/b. (A–C) Dose–response stimulation of MC4R by α−MSH−induced cAMP production in the presence of different amounts of RMrap2a/b (A,B) or RMRAP2 (C). (D–F) Dose–response inhibition of MC4R by antagonist SHU9119 in the presence of different amounts of RMrap2a/b (D,E) or RMRAP2 (F). (G–I) The constitutive activity of MC4R in different dosages of RMRAP2. Data were analyzed by one−way ANOVA and shown as mean ± SEM of three replicates. ns (not significant difference), * p < 0.05, *** p < 0.001 and **** p < 0.0001.