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. 2022 May 29;11(6):834. doi: 10.3390/biology11060834

Figure 3.

Figure 3

Validation of RNA-seq data by qRT-PCR. (A,C,E,F) show the tendencies of mRNAs, lncRNAs, circRNAs, and miRNAs, respectively, in RNA-seq data using the TPM value. (B,D,F,H) represent the relative expressions of candidate mRNAs, lncRNAs, circRNAs, and miRNAs, respectively, by qRT-PCR. The TPM value is represented as the means, while the data of the relative expressions shown are the means ± SEM. The asterisk indicates significant differences. ** p < 0.01.