FIG. 1.

Characteristics of marY1 cloned into λA2. Insert DNA is represented by a solid black line. The thin line represents adjacent λEMBL3 vector DNA. Restriction sites of BamHI (B), BglII (G), EcoRI (E), HindIII (H), KpnI (K), SalI (S), and XhoI (X) are given. The arrow denotes the direction of marY1 and the location of the fragment previously cloned in pHHM145 (18). The solid black bar and the hatched bar underneath the insert DNA indicate probes for the plaque hybridizations used to identify λA2 and Southern hybridizations to determine the distribution of the marY1 homologues in fungal strains, respectively. Domains corresponding to 5′ LTR, 3′ LTR, gag, prt, and pol are given. The location of a putative ribosomal frameshifting site is indicated by the arrowhead. A zinc-finger DNA-binding site in the gag gene product, a consensus catalytic site in prt, and domains of reverse transcriptase (RT), RNase H (RH), and integrase (IT) are aligned at the bottom.