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. 2022 May 31;11(6):1101. doi: 10.3390/antiox11061101

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effects of silibinin on mitochondrial function in H₂O₂-exposed SH-SY5Y cells. SH-SY5Y cells were treated with different concentrations of silibinin in the presence of 750 μM H₂O₂ for 12 h. (a) Confocal images showing fluorescence signal of DCF, scale bar = 50 μm. (b) Quantitative analysis of fluorescent intensity in (a) by Image J. (c) Confocal images showing fluorescence signals of MitoSOX, scale bar = 50 μm. (d) Quantitative analysis of fluorescence intensity in (c) by Image J. (e) Confocal images showing fluorescence signals of JC-1, scale bar = 50 μm. (f) Quantitative analysis of fluorescence intensity in (e) by Image J. Data are expressed as mean ± SD of three independent experiments (n = 3). # p < 0.05 and ## p < 0.01, compared with the control group; * p < 0.05 and ** p < 0.01, compared with the H₂O₂-treated group.