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Mechanical compression toward 3D HTM spheroids. (A) Thirty 3D HTM spheroids were mechanically compressed using a micro-squeezer system (Sph: 3D sphenoid, S: pressure sensor, P: compression plate) for 10, 30, or 60 min. (B) Representative immunolabeling image of a 3D HTM spheroid mechanically compressed for 60 min stained with DAPI (scale bar: 100 µm).
