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. 2022 Jun 6;10(6):1338. doi: 10.3390/biomedicines10061338

Figure 5.

Figure 5

mRNA expression of the ECM proteins of the 3D HTM spheroids after a mechanical compression stress for 30 min. In the presence of 5 ng/mL TGF-β2 (TGF) or 250 ng/mL DEX, the 3D HTM spheroids were mechanically compressed on day 6 for 30 min and subjected to a qPCR analysis to estimate the expression of mRNA of the ECM proteins (COL 1, COL 4, COL 6, FN). These data were compared with those of the untreated 3D HTM spheroids compressed for 30 min (as above). All the experiments were performed in duplicate using fresh preparations (n = 4). The data are presented as the arithmetic means ± standard error of the mean (SEM). Note: * p < 0.05, ** p < 0.01, (ANOVA followed by Tukey’s multiple comparison test).