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. 2022 May 28;11(6):1071. doi: 10.3390/antiox11061071

Figure 2.

Figure 2

Effect of the plant extract mixture on the gene expression of inflammation marker in an AD-like in vitro model. NHEKs not treated with specific cytokines were used to reflect healthy skin conditions. These cells were left unstimulated (unstim.) or stimulated with the plant extract (Extract) for 20 h. NHEKs were also treated for 20 h with an AD cytokine mix (IL-22, TNF-alpha, IL-13 and IL-4 (each 10 ng/mL)) either in the absence (AD) or in the presence of the extract (AD + Extract). Gene expression levels of (a) IL-24 (b) CA2 (c) CCL26 were determined by real-time PCR and normalized to the gene expression of the housekeeping gene RP38. Statistical significances were tested by (b,c) Kruskal-Wallis test with subsequent Dunn’s multiple comparison and (a) Welch’s ANOVA with subsequent Dunett´s T3 multiple comparison test. p-values were marked by asterisks: ** p < 0.01 (n = 9–18 stimulations).