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. 2022 Jun 17;11(6):926. doi: 10.3390/biology11060926

Table 3.

Corresponding p-values of linear, interaction, and quadratic terms of regression coefficients obtained by RSM of responses for TPC, TFC, TAC, AA, DPPH, and CUPRAC experiments.

Source DF F-Value p-Value F-Value p-Value F-Value p-Value F-Value p-Value p-Value p-Value F-Value p-Value
TPC (mg GAE/100 mL) TFC (mg CE/100 mL) TAC (mg Cy-3-gly/100 mL) AA (mg/100 mL) DPPH (% Inhibition) CUPRAC (% Inhibition)
Model 5 3896.75 0.00000 10,322.31 0.00000 320.69 0.00000 2152.32 0.00000 152.89 0.00000 90.57 0.000000
Linear 2 5714.21 0.00000 15115.1 0.00000 314.37 0.00000 710.19 0.00000 80.88 0.00000 54.06 0.000000
A 1 145.71 0.00001 370.8 0.00000 563.6 0.00000 492.07 0.00000 40.64 0.00000 27.78 0.001000
B 1 11,424.47 0.00000 30,222.06 0.00000 105.62 0.00000 1051.49 0.00000 133.6 0.00000 88.75 0.000000
Square 2 34.34 0.00024 62.01 0.00000 358.48 0.00000 3234.75 0.00000 161.59 0.00000 83.31 0.000000
A2 1 35.69 0.00056 59.62 0.00000 72.14 0.00000 6420.62 0.00000 219.86 0.00000 114.43 0.000000
B2 1 47.74 0.00023 90.56 0.00000 709.4 0.00000 590.74 0.00000 173.04 0.00000 88.05 0.000000
2-Way Interaction 1 12,459.79 0.00000 32,911.97 0.00000 741.54 0.00000 2704.48 0.00000 173.38 0.00000 119.42 0.000000
A*B 1 12,459.79 0.00000 32,911.97 0.00000 741.54 0.00000 2704.48 0.00000 173.38 0.00000 119.42 0.000000
Error 7
Lack-of-Fit 3 * * * * 2.31 0.21800 * * 2.19 0.23100 * *
Pure Error 4
Total 12
R2 99.96% 99.99% 99.57% 99.93% 99.09% 98.48%
Adj R2 99.94% 99.98% 99.25% 99.89% 98.44% 97.39%
Pred. R2 99.75% 99.90% 96.49% 99.44% 95.66% 89.96%

A: time; B: amplitude; DF: degrees of freedom; TPC: total phenolic content; TFC: total flavonoid content; TAC: total anthocyanin content; AA: ascorbic acid; DDPH: radical scavenging activity; CUPRAC: Cupric reducing antioxidant capacity; GAE: gallic acid equivalent; CE: catechin equivalent; Cy-3-gly: cyanidin 3-O-glucoside. * Doesn’t matter in statistical calculation.