Figure 3.

EpisomiR, a subtype of isomiR with different miRNA methylations. Although not fully understood, the methylation of miRNAs modulates miRNA silencing function and mRNA degradation. Recently developed state-of-the-art sequencing techniques, such as tunnel current sequencing and mass spectrometry analysis, enables the detection of m6A and 5mC modifications. 5mC, 5-methylcytosine; AGO2, argonaute RISC catalytic component 2; DNMT3A, DNA methyltransferase 3 alpha; m6A, N6-methyladenosine; m7G, 7-methylguanosine; METTL1, methyltransferase-like protein 1; METTL3, methyltransferase-like protein 3; miRISC, miRNA-induced silencing complex.