Skip to main content
. 2022 Jun 13;10(6):1397. doi: 10.3390/biomedicines10061397

Figure 6.

Figure 6

The influence of insulin in mediating the effects of LL35 depletion. (A) Insulin tolerance test in vivo. Glucose blood level in mice was measured each 15 min for 1 h after insulin injection (1 U/kg). LL35 ASO—mice with depleted LL35, Luc ASO—control mice. (B) Biochemical parameters of mice blood at day 5 after LL35 depletion (LL35 ASO) in comparison to control mice (Luc ASO). Insulin treatment compensates these differences. (C) Western blot of pAKT1 and AKT1 proteins in AML12 cells after LL35 knockdown (LL35 ASO) and control (Luc ASO) with 100 nM (+Ins) or without insulin treatment. (D) Quantification of pAKT1/AKT1 ratio for AML12 cells after LL35 knockdown and insulin treatment. Results show mean ± SD. ns—not significant. * p < 0.05, ** p < 0.01.