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. 2022 May 25;11(6):811. doi: 10.3390/biology11060811

Figure 1.

Figure 1

Evaluation of SELENOM-KD and SELENOT-KD efficiency by fluorescent microscopy (a,b), real-time PCR (c), and Western blotting (d,e). KD effect on cell proliferation. (a,b) Images of A-172 cell culture in Fura-2 fluorescence (Fura-2) at an excitation of 380 nm and eGFP fluorescence indicating efficiency of expression of the construct transduced into cells. (c) Real-time PCR analysis of mRNA expression of genes encoding SELENOM and SELENOT in A-172 cells transduced with constructs SELENOM-KD (or SELENOT-KD) and scrambled RNA (Scra). (d) Western blot analysis of SELENOM and SELENOT content in samples. (e) Quantification of the content of SELENOM and SELENOT in A-172 cells transduced with constructs SELENOM-KD (or SELENOT-KD) and scrambled RNA (Scra). Comparison of KD groups vs. Scra: *** p < 0.001. (f) Proliferation assay performed by MTT analysis. The optical density at 590 nm was measured, and the values of the respective untreated cells were defined as 100%. Standard deviations were determined by analysis of data from at least three independent experiments and were indicated by error bars. Comparison of KD groups vs. Scra: n/s—data not significant (p > 0.05).