Figure 4.

Effect of SELENOM-KD (a) and SELENOT-KD (b) on the Ca²⁺ capacity of the endoplasmic reticulum. (a,b) Ca²⁺ signals in the Scra, SELENOM-KD (a), and SELENOT-KD (b) experimental groups to the application of thapsigargin (TG, 10 mM) in a nominally calcium-free medium supplemented with Ca²⁺ chelator EGTA (0.5 mM). At the end of the experiment, 10 μM ATP was added as a test for the depletion of the ER Ca²⁺ pool. Ca²⁺ signals of cells in one experiment and their average value are presented. The number of cell passages used for experiments is 4.