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. 2022 May 25;11(6):811. doi: 10.3390/biology11060811

Figure 12.

Figure 12

The effect of SELENOT-KD on the expression of SELENOT partner proteins under ER stress. (a) Comparative analysis of mRNA expression patterns of SELENOT proteins-partners under SELENOT-KD and without SELENOT-KD as well as under conditions of ER stress caused by 0.1 µM MSA or 5 µg/mL SeNP, obtained using real-time PCR. Comparison of experimental groups regarding control, indicated as dash line (1): n/s—data not significant (p > 0.05), * p < 0.05, ** p < 0.01 and *** p < 0.001; (b) comparative analysis of the quantitative content of SELENOT proteins-partners, using Western blotting; (c) quantification of the content of SELENOT proteins-partners in A-172 cells transduced with SELENOT-KD and scrambled RNA (Scra) under conditions of ER stress caused by 0.1 µM MSA or 5 µg/mL SeNP. Comparison of SELENOT-KD vs. Scra: n/s—data not significant (p > 0.05), *** p <0.001.