Table 9.
Time–kill evaluation of Mabs with BoEA and ElAc. Clarithromycin concentration was used at MIC. Growth control: no compound was added to the cell suspension.
| Time (h) | Control Group |
Antibacterial Agents | ||||||
|---|---|---|---|---|---|---|---|---|
| CLA | BoEA MIC |
BoEA 2 × MIC |
BoEA 4 × MIC |
ElAc MIC |
ElAc 2 × MIC |
ElAc 4 × MIC |
||
| 0 | 5.9 ± 0.08 | 5.9 ± 0.08 | 5.9 ± 0.08 ** | 5.9 ± 0.08 ** | 5.9 ± 0.08 ** | 5.9 ± 0.08 ** | 5.9 ± 0.08 ** | 5.9 ± 0.08 ** |
| 12 | 7.03 ± 0.12 | 6.76 ± 0.16 | 6.83 ± 0.12 ** | 6.83 ± 0.12 ** | 6.9 ± 0.08 ** | 6.83 ± 0.12 ** | 6.66 ± 0.23 ** | 5.66 ± 0.12 ** |
| 24 | 8.9 ± 0.08 | 6.73 ± 0.16 | 7.23 ± 0.16 ** | 7.23 ± 0.16 ** | 6.53 ± 0.12 ** | 6.3 ± 0.08 ** | 6.06 ± 0.09 ** | 5.76 ± 0.26 ** |
| 36 | 9.1 ± 0.16 | 6.9 ± 0.08 | 7.4 ± 0.37 ** | 7.1 ± 0.08 ** | 6.33 ± 0.12 ** | 6.53 ± 0.12 ** | 5.83 ± 0.16 ** | 5.73 ± 0.20 ** |
| 48 | 9.76 ± 0.04 | 7.03 ± 0.12 | 8.5 ± 0.40 * | 7.86 ± 0.04 * | 6.66 ± 0.12 * | 6.8 ± 0.08 ** | 5.76 ± 0.04 ** | 5.30 ± 0.08 ** |
| 72 | 10.26 ± 0.44 | 7.5 ± 0.21 | 8.5 ± 0.37 * | 7.23 ± 0.30 * | 6.66 ± 0.12 * | 7.0 ± 0.08 ** | 6.23 ± 0.16 ** | 5.36 ± 0.26 ** |
| 96 | 10.7 ± 0.16 | 8.1 ± 0.14 | 9.7 ± 0.24 * | 8.46 ± 0.41 * | 7.16 ± 0,12 * | 7.36 ± 0.09 ** | 6.23 ± 0.30 ** | 5.33 ± 0.20 ** |
| 120 | 9.7 ± 0.16 | 8.33 ± 0.12 | 9.9 ± 0.08 * | 9.1 ± 0.08 * | 8.33 ± 0.23 * | 6.86 ± 0.12 ** | 7.03 ± 0.12 ** | 6.26 ± 0.18 ** |
Data are presented as the mean ± standard deviation (SD) of Colony-Forming Unit (CFU) and representing of one of three independent experiments performed in triplicate. Statistical analysis was performed using analysis of variance and post hoc t-test. * p < 0.05, ** p < 0.005 when compared to the growth control. Abbreviations: BoEA, ethyl acetate fraction of B. orellana; E1Ac, ellagic acid; MIC, minimal inhibitory concentration.