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. Author manuscript; available in PMC: 2022 Jun 23.
Published in final edited form as: Exp Eye Res. 2021 Apr 15;207:108581. doi: 10.1016/j.exer.2021.108581

Fig. 5.

Fig. 5.

Altered fibroblast and Aspergillus gene expression within the 3D model. A) Periodic Acid Schiff-hematoxylin (PASH) staining of control and A. fumigatus-infected constructs. Fibroblast nuclei stain blue and fungal cell walls stain pink. B) qRT-PCR data demonstrating the upregulation of pro- inflammatory cytokines, chemokines, and matrix metalloprotease genes in Aspergillus- infected (I) constructs relative to uninfected controls (C). C) Milliplex data demonstrating the upregulation of the corresponding proteins. D) qRT-PCR demonstrating the expression of several A. fumigatus collagenase genes grown in the 3D construct model relative to tissue culture media, EMEM media (M). Data for all panels and conditions taken at 48 h post-inoculation.