Skip to main content
. 2022 Jun 6;10(6):1333. doi: 10.3390/biomedicines10061333

Figure 1.

Figure 1

(a) Tail bleeding time after intraperitoneal (i.p.) injection of His-tagged ANGPT2 (A2), ANGPT1 (A1), and IgG with (8 nmol) and high dose (80 nmol) after 15 min or 2 h, as indicated; (b) Plasma concentrations of ANGPT2 after i.p. injections; (c) Plasma concentrations of activated protein C (APC) 15 after high dose injection of indicated proteins; (d) Plasma concentrations of thrombin/antithrombin (TAT) complexes 15 min after high dose injection of indicated proteins; (e) Immunohistochemistry for human/mouse ANGPT2 and TM on lung tissue after administration of high dose of ANGPT2 or IgG for 15 min; (f,g) Representative blot and quantification of His-tag labelled proteins after immunoprecipitation of TM in lung tissue 15 min after intraperitoneal (i.p.) injection of 80 nmol (high dose) of His-tagged ANGPT2 (A2), ANGPT1 (A1), and IgG. Full gels can be seen in the Supplementary Materials, Figure S1a; (h,i) Western blotting and quantification of TM in lung lysates from the same conditions as above. Full gels can be seen in the Supplementary Materials, Figure S1b; (j) Plasma sTM 15 min after injection of 80 nmol ANGPT2 (A2), ANGPT1 (A1), and IgG. Data presented as mean ± 95% CI. Statistical comparison from one-way ANOVA with Bonferroni post hoc test.