Figure 6.

B cell-driven IL-10 production is correlated with macrophage polarization and immunosuppression during SIV infection

(A) Bar plot of numbers of IL-10-positive cells of the indicated types in CN2 and CN8 in all animals.

(B) Box plots of mean IL-10 expression across B cells within the CN2 and across macrophages in CN8. Each dot represents data from a single FOV from SIV-infected and SIV-uninfected controls. Nonpaired Wilcoxon test: ns, not significant; ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.

(C) Plots of Spearman’s correlations between IL-10 quantities and vRNA in CN2 (top left), and CN8 (bottom left), vRNA with the M2 immunosuppressive macrophage marker CD163 in CN8 (top right), and FoxO1 in CN8 (bottom right). Each dot represents data from a single SIV-infected FOV.

(D) Representative pseudocolored MIBI images depicting IL-10, B cell markers (CD20 and Pax-5; top), and macrophage markers (CD68 and CD163; bottom). Two representative FOVs from infected and uninfected animals are shown. The phenotype maps superimposed with IL-10 expression patterns are shown below each MIBI image.

(E) A cartoon depicting a proposed model for B cell-induced immunosuppression of macrophages via IL-10.

See also Figure S6.