Table 6.

Effects of digestive enzymes on Alg NPs + DRS-B2 + lactic acid or Alg NPs + DRS-B2 + menthol antibacterial activities.

MICs of DRS-B2 (µg/mL)
Enzymes Mix	Without Treatment	Treatment with pH Variation Only * (Incubation 30 min at pH 3 and Then 2 h at pH 6)	Treatment with Pepsin * (Incubation 30 min at pH 3 with Pepsin and Then 2 h at pH 6)	Treatment with Digestive Enzymes * (Incubation 30 min at pH 3 with Pepsin and Then 2h at pH 6 with Trypsin + Chymotrypsin)
DRS-B2 (40 µg/mL)	7.5	10	≥10	≥10
Alg NPs (500 µg/mL)	2.5	2.5	5	10
+
DRS-B2 (40 µg/mL)
Alg NPs (500 µg/mL)	1.25	1.25	2.5	2.5
+
DRS-B2 (40 µg/mL)
+
Lactic acid (1 µg/mL)
Alg NPs (500 µg/mL)	1.25	1.25	2.5	2.5
+
DRS-B2 (40 µg/mL)
+
Menthol (10 µg/mL)

* Incubation: 39 °C at 140 rpm. Enzymes were used as follows: Pepsin (15 U/mL); Trypsin (40 U/µL); Chymotrypsin (5 U/mL).