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. 2022 Jun 16;12(6):421. doi: 10.3390/bios12060421

Table 2.

Comparison of specific combinations of SNAs in post-processing of PCR product.

Detection Method Strategy Target Detection Limit Aggregation Time Ref.
Colorimetric As-PCR ssDNA product binds to SNAs. Template DNA 10 Pg Several mins [70]
Colorimetric As-PCR product as a G-quadruplex DNAzyme. Genomic DNA 5.6 fg/μL 10 min [71]
Colorimetric PCR product and SNAs form triplex DNA. Short DNA and long DNA 0.5 pM for short DNA
1.0 Pg/L for long DNA
[74]
Colorimetric T7 exonuclease to treat RT-PCR products. RNA 1 nM 15 min [80]
Colorimetric 5′-exonuclease treat RT-PCR products. RNA 6 copies [81]