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. 2022 May 23;7(10):e149107. doi: 10.1172/jci.insight.149107

Figure 3. TRPA1 and TRPV1 are required for 2- and 4-OHE1–induced DRG activation.

Figure 3

(A) Representative traces show that the GPER antagonist G15 had no effect on 2-OHE1–induced [Ca2+]i responses in DRG neurons. (B and C) Summarized data show that estrogen receptor antagonists (at 1 μM) had no effect on 2-OHE1–induced [Ca2+]i responses in DRG neurons. n = 5 coverslips from 3 mice per group (>150 neurons each). One-way ANOVA; NS, not significant. (D–I) Representative time-lapse traces of 2-OHE1– (D–F) and 4-OHE1–induced (G–I) [Ca2+]i responses in the Trpa1−/−, Trpv1−/−, Trpa1−/−Trpv1−/− DRG neurons. (J–K) Percentage of DRG neurons responding to 2-OHE1 (J) and 4-OHE1 (K) in DRG neurons isolated from WT, Trpa1−/−, Trpv1−/−, and Trpa1−/−Trpv1−/− mice. n = 5 coverslips from 4 mice per group (>200 neurons each). **P < 0.01, ***P < 0.001, ****P < 0.0001, 1-way ANOVA.