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. 2022 May 23;7(10):e149107. doi: 10.1172/jci.insight.149107

Figure 8. Intraplantar injections of 2-OHE1 and 4-OHE1 produce TRPA1-dependent mechanical allodynia in ovariectomized female mice implanted with estrogen pellets.

Figure 8

(A and B) Intraplantar administration of 2-OHE1 (A) and 4-OHE1 (B) induced mechanical allodynia in ovariectomized WT female mice implanted with estrogen pellets in a dose-dependent manner. *P < 0.05, ***P < 0.001, ****P < 0.0001, 2-way ANOVA, n = 5. (C and D) TRPA1-specific inhibitor A967079 but not TRPV1-specific inhibitor AMG9810 reverted mechanical allodynia induced by 3 nmol 2-OHE1 (C) or 4-OHE1 (D). $P < 0.05, $$P < 0.01, $$$P < 0.001, 2-way ANOVA, n = 5. (E and F) Mechanical allodynia induced by 3 nmol 2-OHE1 (E) and 4-OHE1 (F) was markedly reduced in Trpa1−/− but not Trpv1−/− mice. ##P < 0.01, ###P < 0.001, ####P < 0.0001, 2-way ANOVA with Bonferroni’s post hoc analysis, n = 5.