Figure 3.

Immunofluorescence staining for CIV and aggregated $\mathsf{\alpha }$-synuclein in dopaminergic neurons of the SNc. Images show representative illustrations of fluorescence immunoreactivity against the mitochondrial outer membrane (VDAC1, red), CIV (MTCOI, green), aggregated $\mathsf{\alpha }$-syn (5G4, cyan), dsDNA (DAPI, blue), and a combination of all channels (merge). CIV-positive neurons were 5G4-negative (A), or positive with punctate inclusions (B) or LB/PB (C). CIV-negative neurons were either 5G4-negative (D), or positive with punctate inclusions (E). Neuromelanin is marked with an asterisk. Scale bar: 10 microns. The graphs in (F) depict the total percentage of neurons in each $\mathsf{\alpha }$-syn inclusion state (LB or PB/punctate inclusions/no inclusion) within each CIV state (positive/negative) from each of the observers.