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. 2022 Jun 8;7(11):e149741. doi: 10.1172/jci.insight.149741

Figure 2. P210-FITC uptake by mouse bone marrow–derived DCs.

Figure 2

Confocal microscopy of bone marrow–derived DCs (BMDCs) incubated with (A) FITC only or (B) P210-FITC. Same magnification in A and B and red bar = 5 μm in B. (C) FITC internalization was quantified using flow cytometry of CD11c-stained cells. Cells were size gated and then gated on CD11c (C, top panel). CD11c+ cells were then analyzed on CD11c/FITC quadrants and the results plotted on a scatter graph indicating the mean percentage of FITC+ cells on the CD11c+ gate (C, bottom panel; n = 8 each). (D) Heparin binds P210-FITC (no heparin n = 12; 100 U heparin n = 10). (E) Proteoglycan inhibitor p-Nitrophenyl β-D-xylopyranoside (pNP-xyl) blocks proteoglycan-mediated uptake of P210-FITC (n = 5 each). *P < 0.05, 2-tailed t test.