Figure 7. ApoB_KTTKQSFDL pentamer.

(A) Binding scores of P210 epitope sequences listed in Table 2 from REVEAL binding assay. Representative plot of PBMCs from an HLA-A*02:01⁺ volunteer stained with (B) HLA-A*02:01 control pentamer or (C) ApoB_KTTKQSFDL pentamer, (D) with backgating in magenta. (E) ApoB_KTTKQSFDL pentamer⁺CD8⁺ T cells in PBMCs of HLA-A*02:01⁺ volunteers compared with control HLA-A*02:01 pentamer (n = 10). (F) Aliquots available from 8 of the same volunteers were stimulated with 20 μg/mL P210 peptide or vehicle (sterile double-distilled H₂O) for 5 days. Representative scatterplot of vehicle (G) or P210 peptide (H) sample stained with ApoB_KTTKQSFDL pentamer. (I) The P210-stimulated samples were also stained with HLA-A*02:01 control pentamer as reference for pentamer specificity. *P < 0.05 by 2-tailed t test.