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. 2022 Jun 8;7(11):e149741. doi: 10.1172/jci.insight.149741

Figure 8. HLA-A*02:01 transgenic mouse model.

Figure 8

(A) Functional test of transgene in A2Kb-Tg ApoE–/– mice immunized with A2V7 and the detection of A2V7 pentamer+CD8+ T cells. (B) Representative scatterplot of A2V7 pentamer+CD8+ T cells in adjuvant- or A2V7-immunized mice with backgating in magenta. (C) Representative photographs of oil red O–stained en face aortas from female and male A2Kb-Tg ApoE–/– mice fed normal chow (NC) or high cholesterol diet (HC) for 8 or 16 weeks starting at 9 weeks of age. (D) Aortic atherosclerosis in female and (E) male mice at 17 and 25 weeks of age. (F–I) CD4+ memory T cells and (J–M) CD8+ memory T cells in A2Kb-Tg ApoE–/– mice. (N) HLA-A*02:01-P210 pentamer+CD8+ T cells in splenocytes of 17-week-old A2Kb-Tg ApoE–/– mice and (O) in plaques of mice aged >63 weeks old after 4 weeks of HC diet feeding; n = 4. T test for 2-group comparison; 1-way ANOVA with Holm-Šidák multiple comparisons test for more than 2 groups. Number of mice in each group is represented by the number of dots in individual figure. *P < 0.05; †P = 0.06.