Figure 1.

Proteogenomic-based identification of the human leukocyte antigen (HLA) immunopeptidome: (A) Schematic representation of experimental and bioinformatic workflow for HLA-I-presented peptide identification; (B) Distribution of peptide-spectrum match (PSM) scores reported by pNovo3 for peptides identified by all three items of software; (C) Venn diagrams showing the reproducibility of HLA-I-presented peptides identified using the three items of software; (D) Length distribution of HLA-I-presented peptides; (E) Precursor charge distribution of the HCT116 immunopeptidome; and (F) Peptide-spectrum match (PSM) count distribution of the HCT116 immunopeptidome.