Figure 6. FXR agonist treatment increases GLP-1⁺ EEC number and density.

(A–H) Two potent FXR agonists, GW4064 (GW; A–D), or fexaramine (Fex; E–H) alone or in combination with Z-guggulsterone (Z-gg; 10 μM), were administered to Gcg^Tomato mouse–derived enteroids for 24 hours. (A and E) Representative images of enteroids treated with vehicle (Veh), either GW (A, 20 μM) or Fex (E, 20 μM) alone, or either GW (A, 20 μM) or Fex (E, 20 μM) in combination with Z-gg for 24 hours. The average enteroid area (B and F), GLP-1⁺ cell number per enteroid (C and G), and GLP-1⁺ cell number per enteroid area (GLP-1⁺ cell density; D and H) were measured. Scale bar: 100 μm. n = 6 wells/group, each well contains 20 enteroids. Mean ± SEM. Statistics, ANOVA. *P < 0.05, **P < 0.01.