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. 2022 Jun 8;7(11):e157788. doi: 10.1172/jci.insight.157788

Figure 5. Immunotherapeutic blockade of IL-1β increases activated T cells’ infiltration.

Figure 5

(A) Total inflammatory cell and lineage-specific leukocyte numbers from BALFs of 18-week-old CC-LR mice treated with IgG or anti–IL-1β Ab (n = 4–5). (B) Representative flow cytometry analysis of neutrophils (CD11b+Ly6G+) and PMN-MDSCs (CD11b+Ly6CloLy6G+) in BALFs. (C and D) Quantification percentage of (C) neutrophils and (D) PMN-MDSCs in BALFs (n = 5–6). (E–L) Representative flow cytometry analysis and quantification percentage of CD3+PD-1+ T cells (E and F), CD8+ T cells (G and H), IFN-γ–expressing CD8+ T cells (I and J), and granzyme B–expressing CD8+ T cells (K and L) in whole lungs of 18-week-old CC-LR mice treated with IgG or anti–IL-1β Ab (n = 3–6). (M–O) Relative mRNA expression of Ym1 and Mrc1 (M), Cd8a (N), and Pdcd1 (O) in the whole lung, normalized to Cd45 or Actb expression (n = 3–5). (P and Q) MFI of TNF-α (P) or granzyme B (Q) measured by multiplex ELISA in IgG or anti–IL-1β–treated mice (n = 4–5). Data represent mean ± SEM. **P < 0.01, *P < 0.5 by unpaired t test. a IL-1β, anti–IL-1β; Lym, lymphocytes; Mac, macrophages; Neu, neutrophils.