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. 2022 Jun 11;11(12):1894. doi: 10.3390/cells11121894

Figure 4.

Figure 4

Preservation of Mitofilin in RIP3−/− mice is associated with decreased mitochondrial damage and dysfunction after renal I/R. (A) Immunoblots showing dramatic reductions in Mitofilin levels after renal I/R compared to sham mitochondria in WT mice. However, in RIP3−/− mice, the levels of Mitofilin were higher when compared to littermate WT mice after renal I/R. Note that there was no difference in the levels of Mitofilin between WT and RIP3−/− sham mice. Values are expressed as means ± SEM; * p < 0.05 versus WT group; # p < 0.05 versus WT-I/R group (n = 4/group). (B) Images and graph of kidney tissues labeled with Mitofilin showing a dramatic reduction in Mitofilin signaling after renal I/R compared to sham mitochondria in WT mice. However, in RIP3−/− mice, the level of Mitofilin signaling was higher when compared to littermate WT mice after renal I/R. Note that there was no difference in the levels of Mitofilin signaling between WT and RIP3−/− sham mice. Values are expressed as means ± SEM; * p < 0.05 versus WT group; # p < 0.05 versus WT-I/R group (n = 4/group). (C) Immunoblots showing dramatic reductions in Oxphos (electron transport chain complex) levels after renal I/R compared to sham mitochondria in WT mice. Note the preservation of the levels of Oxphos complexes in RIP3−/− mitochondria compared to WT mitochondria after renal I/R.