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. 2022 Jun 11;11(12):1894. doi: 10.3390/cells11121894

Figure 9.

Figure 9

Mitofilin knockdown in HK-2 cells increases the levels of cGAS and STING, with no effect on RIP3 levels. (A) Immunoblots showing increases in the levels of cGAS and STING in HK-2 cells transfected with Mitofilin siRNA compared to those transfected with scrambled siRNA (n = 3 experiments). (B–D) Graph showing increases in the levels of cGAS (D) and STING (C) and reductions in Mitofilin (B) levels in HK-2 cells transfected with Mitofilin siRNA compared to those transfected with scrambled siRNA. Values are expressed as means ± SEM; * p < 0.05 versus control (Ctrl) vector group (n = 3/group). (E) Immunoblots showing no difference in the levels of RIP3 in HK-2 cells transfected with Mitofilin siRNA compared to those transfected with scrambled siRNA (n = 3 experiments). (F,G) Graph showing reductions in Mitofilin (F) levels and no change in RIP3 levels (G) in HK-2 cells transfected with Mitofilin siRNA compared to those transfected with scrambled siRNA. Values are expressed as means ± SEM; * p < 0.05 versus control (Ctrl) vector group (n = 3/group).