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. 2022 May 23;7(10):e158856. doi: 10.1172/jci.insight.158856

Figure 5. EPO alters monocyte gene expression profiles.

Figure 5

(A) Experimental design. (B) Volcano plot of NanoString gene expression depicting differentially expressed genes (yellow) by Limma test (upregulated to the right) for monocytes isolated from B6 mice injected with BALB/c spleen cells/CTLA4-Ig/EPO versus BALB/c spleen cells/CTLA4-Ig, n = 4/group. Selected upregulated or downregulated genes are annotated in red or blue, respectively; x axis fold-change, vertical lines drawn at 1.5-fold; y axis adjusted P value, horizontal line drawn at adjusted P value = 0.05. (C) Multigroup heatmap demonstration of key upregulated or downregulated genes. (D) Bar chart of significantly enriched biological processes of differentially upregulated or downregulated genes comparing BALB/c spleen cells/CTLA4-Ig/EPO with BALB/c spleen cells/CTLA4-Ig.