Figure 12. Placental domains and their associated clinical phenotypes lead to clustering of intermediate monocyte gene expression.

There were 3 major groups based on hierarchical clustering of intermediate monocyte gene expression (n = 66). The groups were composed primarily of term infants (Group B), preterm infants with acute chorioamnionitis and placental inflammation (Group C), and preterm infants with MVM and preeclampsia (Group A). There were 3549 differentially expressed genes based on DeSeq2, using FDR < 0.05 and 2-fold change. The genes in Cluster 1 were involved in inflammatory biological processes and cell signaling. The genes in Cluster 2 were involved in cell proliferation and cell adhesion. Cluster 3 contained genes related to cytokine signaling and leukocyte degranulation. Cluster 4 and Cluster 5 contained genes involved in transcription and translational processes.