Figure 6. WGCNA of classical monocytes reveals gene clusters based on GA and mode of delivery.

WGCNA of n = 64 samples revealed ontologies related to immune cell activation, migration, and chemokine response were unregulated in term vaginal deliveries (Module 9 [M9]). Genes related to inflammation and inflammatory response were upregulated in term vaginal and preterm vaginal deliveries and were relatively downregulated in preterm cesarean deliveries (M6 and M7). Genes related to neutrophil activity and granulocytes were upregulated in term preterm vaginal deliveries and downregulated in term vaginal deliveries (M2). Classical monocytes from preterm vaginal deliveries upregulated genes associated with DC interaction compared with monocytes isolated from preterm caesarean deliveries (M3).