TABLE 5.
Miscellaneous SLC and SLCO transporters regulated by miRNAs.
| Transporters | Substrates | miRNAs | Models | Observations | References |
|---|---|---|---|---|---|
| SLC4A4/NBCe1 | Sodium bicarbonate | miR-224-5p | Colon cancer cell line HT29 | Transfection with anti-miR-224 increased SLC4A4 by almost 5-fold | Mencia et al. (2011) |
| miR-224-5p | Enamel mineralization Human samples collected from 15- to 25-week-old fetal cadavers | Twofold increase in ameloblast lineage cells SLC4A4 after transfected with miR-224 inhibitor. About 3.5-fold increase in oral buccal mucosal epithelial cells after transfected with miR-224 inhibitor | Fan et al. (2015) | ||
| miR-223-3p | Clear cell renal cell carcinoma cell lines 786-O, ACHN, Caki-1, kidney cell line HK2 TCGA-KIRC dataset, 24 patient samples Pancreatic cancer cell lines AsPC-1, PANC-1, COLO357, HPDE, and prostate cancer PC-3 cells Gene Expression Omnibus database, GSE79634, GSE41369, GSE91035, and GSE16515 datasets 67 paired pancreatic cancer and normal tissue samples Xenograft mouse model | SLC4A4 was decreased by about 50% with miR-223-3p mimic transfection and increased 2–3-fold with miR-223-3p inhibitor | Xiao et al. (2019) | ||
| Transfection with miR-223-3p mimic reduced SLC4A4 mRNA by about 50%, while transection with miR-223-3p inhibitor increased SLC4A4 mRNA by about 2.5-fold | Zhang et al. (2020) | ||||
| SLC6A1/GAT1 | γ-Aminobutyric acid | miR-200c-3p | Clear cell renal cell carcinoma cell line 786-O 82 paired clear cell renal cell carcinoma and normal tissue samples Orthotopic transplantation into mice | Negative correlation between miR-200c-3p and SLC6A1 mRNA | Maolakuerban et al. (2018) |
| SLC6A2/NET | Norepinephrine Epinephrine Dopamine | miR-579-3p | Samples from unrelated German panic disorder patients HEK293 cell line | Luciferase reporter assay used to confirm the direct regulation of SLC6A2 by miR-579-3p | Hommers et al. (2018) |
| SLC6A3/DAT | Dopamine | miR-137-3p miR-491-5p | Neuroblastoma cell lines SK-N-SH, SK-N-BE (2), and hepatoma cell line HepG2 | Transfection with miR-137 or miR-491 mimic reduced SLC6A3 mRNA by about 60% | Jia et al. (2016) |
| SLC6A4/SERT | Serotonin | Mmu-miR-16-5p | Depression and anxiety 1C11 murine cells and Hela cells male Swiss-Kunming mice | miR-16 reduced [3H]-paroxetine and [125I]-RTI-55 binding sites specific to SLC6A4 by 40%. SSRI fluoxetine (Prozac) upregulates miR-16 to decrease SLC6A4 | Baudry et al. (2010) |
| SLC6A6/TauT | Taurine γ-Aminobutyric acid | miR-3156-3p | HPV-positive cervical cancer cell lines HeLa, CaSki, SiHa and the HPV-negative CC cells C33A and HT-3 cell lines HPV16/18-positive cervical cancer, HPV-negative cervical cancer, and HPV16/18-negative normal cervical samples | Negative correlation of miR-3156-3p and SLC6A6 protein, but not mRNA | Xia et al. (2017) |
| SLC9A9 | Sodium protons | let-7f-5p | Colitis-associated cancer Colorectal adenocarcinoma cell line Caco-2 Male or female C57BL/6 mice | Transfection of let-7f-5p precursor reduced SLC9A9 | Chen et al. (2019) |
| SLC23A1/SCVT1 | L-/D-ascorbic acid Dehydroascorbic acid | miR-103a-3p | Vitamin C transport in intestinal epithelial cells Colorectal adenocarcinoma cell line Caco-2 and colon cell line NCM460 C57BL/6 J male mice | Transfection with miR-103a-3p mimic decreased SLC23A1 mRNA by about 40% and protein by about 30% | Subramanian et al. (2019) |
| SLC23A2/SVCT2 | L-/D-ascorbic acid Dehydroascorbic acid | miR-141-3p miR-200a-3p | Mouse bone marrow stromal cells | Transfection with miR-141-3p inhibitor increased SLC23A2 mRNA by about 2-fold. Transfection with miR-200a-3p inhibitor increased SLC23A2 mRNA by about 50% | Sangani et al. (2015) |
| DRA/SLC26A3 | Chloride | miR-494-3p | Colorectal adenocarcinoma cell line Caco-2 and T-84 cells | Transfection with miR-494 mimic does not downregulate SLC26A3 mRNA but does decrease SLC26A3 protein by 50%–60% | Anbazhagan et al. (2014) |
| PAT1, SLC26A6 | Chloride | miR-125a-5p | Colon adenocarcinoma cell line Caco-2, HT-29, T-84, and SK-CO15 cell lines | Transfection with miR-125a-5p reduced SLC26A6 mRNA by about 49% and protein by 44% | Anbazhagan et al. (2019) |
| SLC30A8/ZnT8 | Zinc ion | miR-143-3p | Glioblastoma cell lines T98G, U87-MG (U87), and A-172 19 clinical glioblastoma samples and five control samples Male and female BALB/c nude mice | Overexpression of miR-143-3p significantly reduces SLC30A8 protein, while miR-143-3p inhibition upregulates SLC30A8 protein | Lozada-Delgado et al. (2018) |
| SLC34A2/NaPi-IIb | Phosphate | miR-939-5p | Gastric cancer cell lines AGS, BGC-823, HGC-27, MGC-803, MNK-45, SGC-7901, and GES-1 cell lines paired gastric cancer and normal tissue samples xenograft mouse model | Transfection with miR-939 mimic downregulated SLC34A2 mRNA by about 50% | Zhang et al. (2017) |
| SLC39A6 | Zinc | miR-192-5p | Hepatocellular carcinoma cell lines Huh-7, SK-Hep-1, SNU-449, MHCC-97L, MHCC-97H HCC-LM3, kidney cell line HEK-293T clinical paired hepatocellular carcinoma and normal tissue samples Male BALB/c mice | miR-192 overexpression downregulated SLC39A6 protein and direct regulation was confirmed by luciferase reporter assay | Lian et al. (2016) |
| SLC52A3/RFVT3 | Riboflavin | miR-423-5p | Intestinal absorption of riboflavin Colon adenocarcinoma cell line Caco-2 and small intestine adenocarcinoma cell line HuTu-80 male C57BL/6 mice | Transfection with miR-423-5p mimic did not affect SLC52A3 mRNA level but decreased protein by about 50% | Lakhan et al. (2017) |