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. 2022 Jun 8;7(11):e157393. doi: 10.1172/jci.insight.157393

Figure 3. Administration of BCG-i.v. triggers expansion of LKS+ BM cells.

Figure 3

(A) Schematic representation of the experimental procedure for Flow cytometry. K18-hACE2 mice were i.v. or s.c. inoculated with BCG. Mice in control group were injected i.v. with PBS. Mice BM cells were collected on day 45 after BCG vaccination or PBS treatment and processed to flow cytometry. (B) Representative FACS blots of BM-LKS+ cells in nonvaccinated (PBS-i.v.), BCG-s.c.–, or BCG-i.v.–vaccinated mice (left to right). (C) The total cell number (right panel) and relative cell percentage (left panel) of BM-LKS+ cells in nonvaccinated (PBS-i.v.), BCG-s.c.–, or BCG-i.v.–vaccinated mice. Data are presented as mean ± SD. Statistical significance was calculated using 1-way ANOVA test (*P < 0.05, **P < 0.01, ***P < 0.001). (D) Representative FACS blots of LT-HSCs, ST-HSCs, and MPPs in nonvaccinated (PBS-i.v.), BCG-s.c.–, or BCG-i.v.–vaccinated mice (left to right). (E) The quantification of the total cell numbers (right panel) and relative cell percentage (left panel) of LT-HSCs, ST-HSCs, and MPPs in nonvaccinated (PBS-i.v.), BCG-s.c.,– or BCG-i.v.–vaccinated mice. Statistical significance was calculated using 2-way ANOVA test (**P < 0.01, ***P < 0.001).