TABLE 2.
PHA contents and monomer compositions of P. oleovorans GPo1000 and the phaD insertion mutant P. oleovorans GPo1001 cultivated on octanoatea
| Organism | Time (h) | Cell dry wt (g liter−1) | PHA (%, wt/wt) | PHA monomer compositionb (mol%)
|
||
|---|---|---|---|---|---|---|
| C6 | C8 | C10 | ||||
| GPo1000 | 23 | 0.74 | 54.9 | 7.7 | 92.3 | <0.1 |
| 27 | 0.77 | 56.1 | 7.7 | 92.3 | <0.1 | |
| 46 | 0.86 | 58.3 | 7.5 | 92.5 | <0.1 | |
| GPo1001 | 23 | 0.44 | 9.6 | 6.0 | 94.0 | <0.1 |
| 27 | 0.42 | 8.9 | 6.4 | 93.6 | <0.1 | |
| 46 | 0.35 | 1.1 | 8.5 | 91.5 | <0.1 | |
| GPo1001/pHAD53 | 23 | 0.79 | 38.9 | 6.3 | 93.5 | 0.2 |
| 27 | 0.94 | 43.8 | 6.5 | 93.3 | 0.2 | |
| 46 | 0.94 | 30.7 | 3.3 | 91.3 | 5.4 | |
a
Cells were cultivated in 0.1NE2 minimal medium with 15 mM octanoate. After 23, 27, and 46 h of cultivation, cells were harvested and lyophilized, and the PHA composition was measured by GC.
b
C6, 3-hydroxyhexanoate; C8, 3-hydroxyoctanoate; C10, 3-hydroxydecanoate.
c
Cells were grown until they reached the exponential phase (OD450 = 0.2) and then induced with 0.5 mM IPTG.