Skip to main content
. 2022 Jun 11;11(12):1898. doi: 10.3390/cells11121898

Figure 3.

Figure 3

The mRNA abundance of placenta-specific proteinsPP13, PAPP-A, and cell cycle regulation RGC32 is altered in the preeclampsia (PE) cell models. (A) Placental protein 13 (PP13), (B) pregnancy-associated plasma protein-A (PAPP-A), and (C) the response gene to complement 32 (RGC32), a gene involved in cell cycle regulation. The placental tissues of normotensive controls (gray bar; n = 16) and women with PE (orange bar; n = 13) were compared. The cultured CTB were placed under normoxia (24 h; gray bar with diagonal lines; n = 9), H/R (6 h intervals each of normoxia and hypoxia for 24 h; light orange bar with diagonal lines; n = 9), and hypoxia (24 h; dark orange bar with diagonal lines; n = 9). The procedures regarding the processing of the placental tissues as well as the isolation of the CTB from the healthy term placentae are described in Materials and Methods. The gene expression was assessed by real-time PCR and normalized to the reference gene YWHAZ. Data are shown as the mean ± S.E.M. and represent the fold change (2−ΔΔCq) of the mRNA expression compared to the control or normoxia. The placental expression patterns between the control and PE were compared using the student’s t-test. For comparison of normoxia with hypoxia and H/R, ANOVA was applied. p < 0.05 was considered statistically significant.