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. 2022 Jun 11;11(12):1898. doi: 10.3390/cells11121898

Figure 4.

Figure 4

The mRNA abundances of genes related to the inflammasome complex in the preeclampsia (PE) models reflect the expression patterns of PE placentae. The gene expressions of (A) Nod-like receptor protein 3 (NLRP3), (B) interleukin 1β (IL-1β), and (C) caspase 1 (CASP1) were assessed by real-time RT-PCR and normalized to the reference gene YWHAZ. The placental tissues of the controls (gray bar; n = 16) and preeclamptic (PE, orange bar; n = 13) pregnancies were compared. The cultured CTB were placed under normoxia (24 h: gray bar with diagonal lines; n = 9), H/R (6 h intervals each of normoxia and hypoxia for 24 h; light orange bar with diagonal lines; n = 9), and hypoxia (24 h; dark orange bar with diagonal lines; n = 9). The procedures regarding the processing of placental tissues as well as the isolation of the CTB from the normotensive control placentae are described in Materials and Methods. Data are shown as the mean ± S.E.M. and represent the fold change (2−ΔΔCq) of the mRNA expression compared to the control or normoxia, respectively. The placental expression patterns between the control and PE were compared using the student’s t-test. For comparison of normoxia with hypoxia and H/R, ANOVA was applied. p < 0.05 was considered statistically significant.