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. 2022 Jun 11;11(12):1898. doi: 10.3390/cells11121898

Figure 7.

Figure 7

The protein secretions of sFlT-1 and PlGF into the supernatants of the preeclampsia (PE) cell models. The protein secretions of (A) sFlT-1 and (B) PlGF were measured using ELISA kits following the manufacturer´s instructions. The protein secretion was measured in the media of the CTB cultured under normoxia (gray bar with diagonal lines), H/R (light orange bar with diagonal lines), and hypoxia (dark orange bar with diagonal lines) conditions. (C) depicts the calculated sFlT-1/PlGF ratio. The gray dashed line (>38 PE) shows the cutoff value for PE as determined in human serum samples and used for diagnostic purposes in the clinical context. The black dash-dot line (>85) marks the cutoff value for early-onset PE (EO-PE), and the black dotted line (>110) depicts the cutoff value for late-onset PE (LO-PE). The results are presented as mean ± S.E.M. (n = 9 different CTB isolations cultured under normoxia, H/R, and hypoxia). Statistical analyses were performed by paired 2-way ANOVA. p < 0.05 was considered statistically significant.