Table 1.
Characteristics of primers.
| Category | Gene | Accession Number | Primer Sequence (5′-3′) |
|---|---|---|---|
| Angiogenic and antiangiogenic markers | FlT-1 | NM_001160030.1 | F-AAGAGCCTGAACTGTATA R-TGATGACGATGATGATGA |
| ENG | NM_001114753.2 | F-CAAGACCAGGAAGTCCATA R-CGTGTGCGAGTAGATGTA |
|
| PIGF | NM_001293643.1 | F-CTCCTAAAGATCCGTTCTG R-CTTTCCGGCTTCATCTTC |
|
| VEGF A | NM_001287044.1 | F-TACATCTTCAAGCCATCC R-TTCTTTGGTCTGCATTCA |
|
| RAS receptors | AGTR1 | NM_032049.3 | F-TCTCAGCATTGATCGATACC R-TGACTTTGGCTACAAGCATT |
| AGTR4 | NM_005575.3 | F-TATGCCTAAGAAGTCATCAG R-CCAAGTAAGTGCTCATCT |
|
| Hypoxia and oxidative stress markers | HIF1α | NM_001530.4 | F-CGTTGTGAGTGGTATTATTC R-GGCTACTTGTATCTTCTGA |
| NRF2 | NM_005654.6 | F-ATCGTGCTGTTCACGTCAGAC R-TGGCTCCTCACGTACTCCTC |
|
| Endoplasmic reticulum (ER) stress markers | GRP78 | NM_005347.5 | F-TGTGCAGCAGGACATCAAGT R-TCCCAAATAAGCCTCAGCGG |
| GRP94 | NM_003299.3 | F-GCCAGTTTGGTGTCGGTTTC R-GGGTAATTGTCGTTCCCCGT |
|
| Placenta-specific proteins | PP13 | NM_013268.3 | F- GATATTGCCTTCCGTTTC R-GTAGTCTGTTGTCTCCTC |
| PAPP-A | NM_002581.5 | F-GCTGTCACATACATCCAT R-GCTGGGTTCATCAATACA |
|
| Cell cycle regulator | RGC-32 | NM_014059.3 | F-ATTCTCCAACAGACTCTAC R-CAAGATCAGCAATGAAGG |
| Syncytial marker | β-hCG | NM_033043.2 | F-CGGGACATGGGCATCCAA R-GCGCACATCGCGGTAGTT |
| Inflammasome | NLRP3 | NM_001079821 | F-CACCTGTTGTGCAATCTGAAG R-GCAAGATCCTGACAACATGC |
| IL-1β | NM_000576 | F-CTGTCCTGCGTGTTGAAAGA R-TTGGGTAATTTTTGGGATCTAC |
|
| CASP1 | NM_033295 | F-GCCTGTTCCTGTGATGTGGAG R-TGCCCACAGACATTCATACAGTTTC |
|
| Reference gene | YWHAZ | XM_024447266.1 | F-CCGTTACTTGGCTGAGGTTG R-AGTTAAGGGCCAGACCCAGT |