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. 2022 Jun 19;10(6):1446. doi: 10.3390/biomedicines10061446

Figure 8.

Figure 8

CDNF affects complexity analysis of Iba1−positive cell morphologies and suppresses the production of proinflammatory cytokines in the grafted striatum at one month after transplantation. (A–D) Fractal analysis of microglia/macrophages in Iba1-stained tissue. Original photomicrographs were subjected to a series of uniform ImageJ plugin protocols prior to conversion to binary images. Binary images were subsequently analyzed by using FracLac of ImageJ, which quantifies single cell complexity (fractal dimension, fD). Representative images of morphological changes in Iba1−staining cells within the left striatum in rVM−grafted rats (A), the right striatum in rVM-grafted rats (B), the left striatum in rVM + rhCDNF−grafted rats (C), and the right striatum in rVM + rhCDNF−grafted rats (D). Scale bar = 20 µm. (E) Summary data and statistical analysis of fractal dimension at one month after transplantation (n = 17−30, one-way ANOVA p < 0.001, *** p < 0.001 compared to the right striatum in rVM-grafted rats, Tukey’s post hoc test). (F) IL-1β concentrations in the grafted striatum were measured by ELISA. (G) TNF−α concentrations in the grafted striatum were measured by ELISA. (H) IL−6 concentrations in the grafted striatum were measured by ELISA. * p < 0.05, ** p < 0.01 indicates comparison with rVM group with Student’s t-test. The data represent mean ± SEM.