Figure 8.

The basal level of CHKA, CERK, and SMPD1 mRNA in different PCa cell lines: (A) Quantification of CHKA, CERK, and SMPD1 by qPCR. Data were normalized to GAPDH expression. Data are represented as the mean of 2-ΔCt ± SEM (n = 3). * p < 0.05. (B) The antitumoral effect of MALAT1 targeting. Schematic cartoon showing the role of MALAT1 in androgen receptor signaling in advanced prostate cancer. The metabolic genes CHKA and CERK are transcriptionally regulated by androgen/androgen receptor (AR) on Androgen Responsive Element (ARE) sites (Left). MALAT1 reduces AR activity by forming a MALAT1/AR complex without androgens. MALAT1 depletion enables unliganded-AR to regulate CHKA and CERK genes, exerting anti-tumoral activity (Right).