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Fluorescence microscopy for 5-ALA and lectin. (A) In low-grade gliomas (Case #2 on Table 1), 5-ALA fluorescent cells (purple) can be found occasionally within vascular structures (green). Scale bar, 20 μm. (B) In high-grade gliomas (Case #5 on Table 1), several 5-ALA fluorescent cells (purple) are seen outside blood vessels (green) whose wall appears discontinuous. Scale bar, 30 μm. Objective lens, Olympus PlanApo N 60×/1.42 Oil.
