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The residue E354 is important for luciferase sensitivity to Zinc. Effect of 2 mM zinc on the bioluminescence spectra of: (A) Macrolampis sp2 wild-type; (B) C. distinctus wild-type; (C) C. distinctus E354N, and (D) Macrolampis sp2 N354E mutant (black line) without zinc, and (gray lines) in the presence of zinc. Reprinted with permission from ref. [33]. Copyright 2016 Springer.
